This track displays CpG methylation data generated from Oxford Nanopore Technology (ONT) long-read sequencing. Values represent the fraction of reads supporting 5-methylcytosine (5mC) modification at each CpG site, ranging from 0 (unmethylated) to 100 (fully methylated).
Raw ONT reads were basecalled and methylation-called using dorado (v0.5.3)[sup4.3.0+5mCG_5hmCG]. Reads were aligned to matched assembly using minimap2 (v2.28)[--mod-thresholds m:0.8 --filter-threshold C:0.5]. Methylation pileups were generated using modkit pileup (v0.4.2) and converted to bigWig format using bedGraphToBigWig (v2.10).
The y-axis represents the percent of aligned CpG's with 5mC modification at a given CpG site (0–100). Only CpG-context methylation is shown.
The raw data and processed files are available at [https://github.com/ph09/t2t_marmoset].
Data generated by Julian Menendez, Miga Lab, UCSC [Biomolecular Engineering and Bioinformatics].