Description

This track shows the individual summits from a large scale ChIP-SEQ study described in Tsankov et al., "Transcription factor binding dynamics during human ES cell differentiation", Nature 2015. In this work, the authors performed ~38 ChIP-SEQ of transcription factors in stem cells at different stages of differentiation (pluripotent state, endodem, mesoendoderm, etc).

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Methods

We obtained the sequencing reads from NCBI GEO GSE61475. Bowtie2 2.3.2 was run with in single-end mode and with --end-to-end --sensitive. MACS 2.1.1.20160309 was run with --keep-dup all and no input/control read file.

References

Tsankov AM, Gu H, Akopian V, Ziller MJ, Donaghey J, Amit I, Gnirke A, Meissner A. Transcription factor binding dynamics during human ES cell differentiation. Nature. 2015 Feb 19;518(7539):344-9. PMID: 25693565; PMC: PMC4499331

Email max@soe.ucsc.edu or jferna10@ucsc.edu